Differentiation of apoptotic and necrotic cells in suspension cultures of Taxus cuspidata by the combined use of fluorescent dying and histochemical staining methods

Abstract

A method to differentiate apoptotic and necrotic cells in suspension cultures of Taxus cuspidata was developed by the combined use of the double-fluorescence dying and histochemical staining methods. The cultured cells were directly detected without the operations such as the removal of cell wall, fixing and section of cells as required by other conventional methods. Besides, the living cells, apoptotic and necrotic cells were simultaneously differentiated under a fluorescence microscope and the typical apoptotic morphological features could be observed throughout the whole process. The advantages of this method include simple operation, quick response and high accuracy.