Abstract
Thiobacillus acidophilus andT. ferrooxidans were separated by centrifugation on the basis of their cell density in Renografin gradients. For both species, growth history was the largest factor influencing cell density. Density was greatest forT. acidophilus andT. ferrooxidans grown with tetrathionate, followed byT. acidophilus grown with glucose.T. ferrooxidans grown with ferrous sulfate was the least dense.T. acidophilus was isolated from iron-grownT. ferrooxidans by separation in a Renografin gradient. Plasmid patterns ofT. acidophilus andT. ferrooxidans were used to confirm the separation of the two species in mixed gradients.
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