Abstract
Insulin and prostaglandin F 2 (PGF 2α) stimulate undine and 2-deoxy- d-glucose transport by 3T3-L1 and 3T3-C2 cells. Maximal stimulation of both transport systems is achieved with 1.5 μM PGF 2α. Maximal stimulation of uridine transport is achieved with 34 nM (200 ng/ml) insulin. The basal (control) level of uridine uptake is lower in 3T3-L1 than in 3T3-C2 cells, while the basal level of 2-deoxy- d-glucose uptake is slightly higher in 3T3-L1 cells. The stimulatory effect of PGF 2α on both transport systems is abolished in the course of differentiation of 3T3-L1 cells to adipocytes. Removal of the inducers of differentiation (insulin plus indomethacin) from the medium of differentiated cells does not restore responsiveness of either transport system to PGF 2α. In contrast the stimulatory effect of insulin on either transport system is not abolished during differentiation. Treatment of the non-differentiating 3T3-C2 cell line with inducers of differentiation reduces the basal level of 2-deoxy- d-glucose transport by 70% and only slightly decreases the basal level of uridine transport without affecting the stimulatory effect of PGF 2α on either transport system.
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