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Abstract
Dendritic cells (DCs) are the most potent antigen-presenting cells that play a critical roles in connecting innate and adaptive immunity. Allhough many studies about mouse and human DCs have been performed, those using other mammalian species including domestic animals are lacking. ln this study, we established an efficient experimental method for swine myeloid-origin DCs. Bone marrow cells (BMs) were cultured in vitro using an adequate cytokine for this purpose. BMs were harvested from bones of piglets and cultured in the presence of porcine recombinant granulocyte-monocyte colony stimulating factor (GM-CSF) for 6-10 days. And also, we tried to find optimal conditions for cryopreservation of BMs. After 6 day culture, semi-floating cells were harvested and characterized as DCs (BMDCs). Interestingly, BMDCs revealed the excellent production of TNF-alpha, a representative innate cytokine, upon Iipopolysaccharide exposure, but not the case of nitric oxide. In addition, we measured the expression level of immunological markers on the surface of BMDCs. Taken together, we established an efficient experimental method for swine myeloid-origin DCs. The information obtained from this study includes optimal culture conditions and thus may help researchers studying swine immunity in basic scientific and-clinicalfields.
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