Differentially Expressed Genes in Oleic Acid Synthesis of <I>Brassica napus</I> by Detected Gene Chip

Abstract

Oil from rapeseed with high oleic oil content is insensitive to oxidation and long shelf life.In daily diet with high oleic acid oil can reduce low-density lipoprotein cholesterol levels in blood and prevent arteriosclerosis.In addition,high oleic acid content in edible oil is an important fat indicator.Oleic acid can be effectively converted to methyl ester,which is beneficial to produce biodiesel.Oleic acid content in conventional rapeseed is only 17%.However,oleic acid content in low erucic acid rapeseed reaches around 60%.Differential expressed genes of oleic acid have been found in the high oleic acid line(71.71%) and low oleic acid line(55.6%) of Brassica napus by gene chip technique.The results showed that there were 562 differential expressed genes relating to high and low oleic acid contents in rapeseed,among them 194 were up-regulated genes and 368 were down-regulated genes.The up-regulated gene NM_100489 and the down-regulated gene NM_130183 were then taken as the materials.Real time RT-PCR was further used to confirm the results of gene chip,and the results of real time RT-PCR analysis agreed with the results by gene chip.According to the results of gene chip,562 differentially expressed genes were annotated functionally using Go Annotation System.The molecular function of differential expressed genes mainly included catalysis of enzymes,binding,transcriptional regulation,metabolism and others with unknown functions.Some genes related to sugar metabolism and fatty acid synthesis were identified as differential expressed genes.They are pyruvate kinase,fructose diphosphate,acyl transfer/acyl-ACP S lipase,hydrolase acting on ester bond,Δ9 stearoyl acid-N-carrier protein desaturase(ADS1),Δ9 acyl-lipid desaturase 2(ADS2),ω-3 fatty acid desaturation enzymes(fad3) and so on.