Differentially Expressed Genes Associated with the Cabbage Yellow-Green-Leaf Mutant in the ygl-1 Mapping Interval with Recombination Suppression.

Xiaoping Liu,Zhiyuan Fang,Yumei Liu,Zhansheng Li,Honghao Lv,Limei Yang,Zhiyuan Li,Xing Li,Yangyong Zhang,Hailong Yu,Mu Zhuang,Fengqing Han

doi:10.3390/ijms19102936

Abstract

Although the genetics and preliminary mapping of the cabbage yellow-green-leaf mutant YL-1 has been extensively studied, transcriptome profiling associated with the yellow-green-leaf mutant of YL-1 has not been discovered. Positional mapping with two populations showed that the yellow-green-leaf gene ygl-1 is located in a recombination-suppressed genomic region. Then, a bulk segregant RNA-seq (BSR) was applied to identify differentially expressed genes (DEGs) using an F3 population (YL-1 × 11-192) and a BC2 population (YL-1 × 01-20). Among the 37,286 unique genes, 5730 and 4118 DEGs were detected between the yellow-leaf and normal-leaf pools from the F3 and BC2 populations. BSR analysis with four pools greatly reduced the number of common DEGs from 4924 to 1112. In the ygl-1 gene mapping region with suppressed recombination, 43 common DEGs were identified. Five of the DEGs were related to chloroplasts, including the down-regulated Bo1g087310, Bo1g094360, and Bo1g098630 and the up-regulated Bo1g059170 and Bo1g098440. The Bo1g098440 and Bo1g098630 genes were excluded by qRT-PCR. Hence, we inferred that these three DEGs (Bo1g094360, Bo1g087310, and Bo1g059170) in the mapping interval may be tightly associated with the development of the yellow-green-leaf mutant phenotype.

Highlights

Yellow-green-leaf mutants have been extensively studied in many species, including Arabidopsis thaliana [1], barley [2], Brassica napus [3], rice [4,5,6], cabbage [7], and muskmelon [8]
Leaf color mutants are an ideal model for studying mechanisms of photosynthesis and light morphogenesis, since yellow-green-leaf mutants are commonly related to chlorophyll synthesis or degradation [9,10]
Runge et al [12] isolated and classified some chlorophyll-deficient xantha mutants of Arabidopsis thaliana and found that some of the mutants were blocked at various steps of the chlorophyll pathway between aminolevulinic acid (ALA) and protochlorophyllide (Pchlide), and the latter did not accumulate in the dark

Summary

Introduction

Yellow-green-leaf mutants have been extensively studied in many species, including Arabidopsis thaliana [1], barley [2], Brassica napus [3], rice [4,5,6], cabbage [7], and muskmelon [8]. Leaf color mutants are an ideal model for studying mechanisms of photosynthesis and light morphogenesis, since yellow-green-leaf mutants are commonly related to chlorophyll synthesis or degradation [9,10]. Leaf color mutants commonly result from blocking a portion of the chlorophyll synthesis pathway, such as the synthesis of 5-aminolevulinic acid (ALA) [11]. Runge et al [12] isolated and classified some chlorophyll-deficient xantha mutants of Arabidopsis thaliana and found that some of the mutants were blocked at various steps of the chlorophyll pathway between ALA and protochlorophyllide (Pchlide), and the latter did not accumulate in the dark

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Discussion

Conclusion