Abstract
Investigators demonstrated some years ago that translation of messenger RNAs (mRNAs) in vitro is characterized by utilization of individual messages with different efficiencies, but the basis of this translational discrimination is poorly understood.1,2 In addition to the in vitro studies, several laboratories have reported competitive mRNA translation in vivo.3–5 Two basic models may be proposed to explain, simplistically, the basis of translational discrimination.1 The first model states that mRNA selectivity is provided by translation components that are mRNA-specific, while the second model states that discrimination occurs as a consequence of differences in affinity among mRNAs for a nonspecific component of the translational system. There are some data that support the first model6; however, the majority of data give support to the second model and strongly suggest that the most significant site of regulation is at the level of initiation of protein synthesis.1,2,4,7
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