Abstract
Polyploidy or genome doubling (i.e., the presence of two or more diploid parental genome sets within an organism) are very important in higher plants. Of particular interest are the mechanisms in the new microenvironment of the common nucleus, where doubled regulatory networks interact to generate a viable genetic system capable of regulating growth, development and responses to the environment. To determine the effects of whole genome merging and doubling on the global gene expression architecture of a new polyploid, derived from protoplast fusion of the A1A1 genome of Gossypium arboreum and the E1E1 genome of Gossypium stocksii, we monitored gene expression through cDNA-AFLP in the somatic hybrids (G. arboreum + G. stocksii). The genomic expression patterns of the somatic hybrids revealed that changes in expression levels mainly involved regulatory genes (31.8% of the gene expression profiles), and the AA and EE genomes contributed equally to genome-wide expression in the newly formed AAEE genome from additivity and dominance effects. These results provide a novel perspective on polyploid gene regulation and hint at the underlying genetic basis of allopolyploid adaption in the new microenvironmental nucleus.
Highlights
The genus Gossypium currently consists of approximately 45 diploid species that are divided into eight monophyletic groups, each designated by a single letter (“A-G. stocksii (Gs)” and “K genome”) and 6 polyploid species[1]
Each of the selective AFLP primer combinations amplified between 100 and 600 fragments, most of which were between 100 and 300 base pairs long. complementary DNA (cDNA)-AFLP analysis using 64 primer combinations resulted in the identification of more than 6800 clear and unambiguous differentially expressed transcript-derived fragments (TDFs) (Fig. 2)
TDF113 (Cotton_A_21823) was annotated as Acyl-CoA N-acyltransferase, the same gene was found in the male sterility mutant of cotton described in our previous paper[44]
Summary
The genus Gossypium (cotton) currently consists of approximately 45 diploid species that are divided into eight monophyletic groups, each designated by a single letter (“A-G” and “K genome”) and 6 polyploid species[1]. Two of the descendant allopolyploid species: Gossypium hirsutum (A1A1D1D1) and Gossypium barbadense (A2A2D2D2), as well as two African-Asian A diploids-Gossypium herbaceum (A1A1) and Gossypium arboreum (A2A2), were each independently domesticated for their long, spinnable, epidermal seed trichomes These four species collectively account for the world’s cotton fiber production, more than 90% of which is provided by upland cotton G. hirsutum[1]. Regardless of the growth stage, tissue, or stress, the degree of bias between duplicated gene pairs is distributed across a spectrum of different expression ratios including the 50:50 ratio of most homoeologous gene pairs[18, 25, 26]. Another consequence of polyploidization is expression level dominance. Expression level dominance has been observed in other polyploid species such as Coffea[27], Spartina[6], and wheat[7]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
