Differential Striatal Axonal Arborizations of the Intratelencephalic and Pyramidal-Tract Neurons: Analysis of the Data in the MouseLight Database.

Kenji Morita,Sanghun Im,Yasuo Kawaguchi

doi:10.3389/fncir.2019.00071

Abstract

There exist two major types of striatum-targeting neocortical neurons, specifically, intratelencephalic (IT) neurons and pyramidal-tract (PT) neurons. Regarding their striatal projections, it was once suggested that IT axons are extended whereas PT axons are primarily focal. However, subsequent study with an increased number of well-stained extended axons concluded that such an apparent distinction was spurious due to limited sample size. Recent work using genetically labeled neurons reintroduced the differential spatial extent of the striatal projections of IT and PT neurons through population-level analyses, complemented by observations of single axons. However, quantitative IT vs. PT comparison of a large number of axons remained to be conducted. We analyzed the data of axonal end-points of 161 IT neurons and 33 PT neurons in the MouseLight database (http://ml-neuronbrowser.janelia.org/). The number of axonal end-points in the ipsilateral striatum exhibits roughly monotonically decreasing distributions in both neuron types. Excluding neurons with no ipsilateral end-point, the distributions of the logarithm of the number of ipsilateral end-points are considerably overlapped between IT and PT neurons, although the proportion of neurons having more than 50 ipsilateral end-points is somewhat larger in IT neurons than in PT neurons. Looking at more details, among IT subpopulations in the secondary motor area (MOs), layer 5 neurons and bilateral striatum-targeting layer 2/3 neurons, but not contralateral striatum-non-targeting layer 2/3 neurons, have a larger number of ipsilateral end-points than MOs PT neurons. We also found that IT ipsilateral striatal axonal end-points are on average more widely distributed than PT end-points, especially in the medial-lateral direction. These results indicate that IT and PT striatal axons differ in the frequencies and spatial extent of end-points while there are wide varieties within each neuron type.

Highlights

There exist two major types of striatum targeting neurons in the neocortex, intratelencephalic (IT) neurons, which project only to telencephalic regions, and pyramidal-tract (PT) neurons, which project out of telencephalon (Wilson, 1986, 1987; Cowan and Wilson, 1994; Levesque et al, 1996; Parent and Parent, 2006; Reiner et al, 2010; Shepherd, 2013)
We examined how the number of intra-striatal axonal end-points is distributed across neuron types as well as across individual neurons
Comparing the ipsilateral axonal end-points between PT and IT neurons, the proportion of neurons having more than 50 ipsipoints within those having at least one ipsi-point is somewhat larger in IT neurons (48/145 = 0.33) than in PT neurons (5/33 = 0.15) (χ2 test, p = 0.042, φ = 0.15)

Summary

Introduction

There exist two major types of striatum targeting neurons in the neocortex, intratelencephalic (IT) neurons, which project only to telencephalic regions, and pyramidal-tract (PT) neurons, which project out of telencephalon (Wilson, 1986, 1987; Cowan and Wilson, 1994; Levesque et al, 1996; Parent and Parent, 2006; Reiner et al, 2010; Shepherd, 2013) These neuron types commonly exist in neocortical areas including the motor cortices, and have distinct, albeit overlapped, layer distributions. Subsequent study from the same laboratory examined an increased number of well-stained extended axons (10 IT neurons and 6 PT neurons), and concluded that such an apparent difference in the axonal morphology was spurious due to limited sample size (Zheng and Wilson, 2002)

Methods

Results

Conclusion