Abstract
Background. Donor dendritic cells migrate into the recipient spleen after hepatic transplantation. We previously reported that immunologic unresponsiveness to rat hepatic allografts can be induced by prior donor-specific blood transfusion (DST). We investigated the phenotype and splenic distribution of donor dendritic cells after allografting and DST.Methods. Donor dendritic cells were identified with anti-rat dendritic cell (OX-62) and anti-donor class II MHC (RT1Ba) (OX-76) antibodies. The phenotype of dendritic cells was determined with antibodies to CD45RC, CD62L, and the maturation markers CD80 (B7-1) and CD86 (B7-2). The cytokine profile of sorted CD45RC+ OX-62+ and CD45RC− OX-62+ dendritic cells was analyzed by reverse transcription polymerase chain reaction (RT-PCR).Results. Pretransplant DST significantly prolonged rat hepatic allograft survival. Immunostaining revealed OX76+/OX-62+ cells in the splenic red pulp of animals receiving pretransplant DST and in the white pulp of untreated animals after transplantation. The ratio of splenic CD45RC− OX-62+ cells to CD45RC+ OX-62+ cells was significantly higher in DST recipients than in untreated animals. CD62L, CD80, and CD86 were lower on CD45RC− OX-62+ than CD45RC+ OX-62+ cells. RT-PCR revealed that sorted CD45RC− OX-62+ cells expressed interleukin (IL)-4 and IL-10. In contrast, sorted CD45RC+ OX-62+ cells expressed only IL-2 and interferon γ (IFN-γ).Conclusion. Differential splenic migration of CD45RC− dendritic cells is associated with immunologic unresponsiveness to rat hepatic allografts.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.