Abstract
The in vivo luminescence responses to eight different mutagenic chemicals were assessed in Photobacterium phosphoreum. The mutagenic effects of these compounds were determined with an N-methyl- N′-nitro- N-nitrosoguanidine (MNNG) induced dark mutant. Treatment of this mutant with compounds known to attack the guanine sites in DA (4-nitroquinoline 1-oxide, mitomycin C and MNNG) stimulated in vivo bioluminescence by a factor of 1×10 3 above control levels after a 10 h exposure period. Bioluminescence was induced by the intercalating agents, ethidium bromide (detectable after 12 h treatment) and 9-aminoacridine (detectable only after streaking treated cultures onto agar plates). No significant induction of bioluminescence was found for hydrazine, novobiocin or 2-aminobiphenyl. Cytotoxic concentrations of these chemicals were measured by the diminution of light output in a bioluminescent strain of P. phosphoreum.
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