Abstract
The posterior pole cytoplasm of early embryos ofDrosophila melanogaster was irradiated with increasing doses of U.V. light at a wave length specific for nucleic acids 35 and 75 min after ovoposition, when nuclei were still inside the yolk mass. Probit analysis showed that:Sterility was higher in irradiations performed at 35 min.Mortality was higher in the experiment performed at 75 min.After treatment at 35 min, sterility and mortality were independent phenomena.The numbers of targets involved in germ cell determination were of the order of tens and hundreds at 35 and 75 min respectively.The numbers of targets involved in viability were of several orders of magnitude in both developmental stages.The irradiation, even at very low doses, caused a background effect which was probably due to "old" embryos. The sex ratio was distorted in favour of females at high doses and of males at low doses.
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