Abstract
Differences in susceptibility of alfalfa genotypes to Sclerotinia sclerotiorum and S. trifoliorum were evaluated by comparing the extent of pathogenesis in individually inoculated stems of replicated clones. Stems were inoculated by applying cotton pads, saturated with 20% V-8 juice and infested with each pathogen, around tips and enclosing with tape. Plants were incubated in a saturated atmosphere for 3 days to establish infection and then in ambient air for 11 days. Pathogenesis proceeded basipetally within stems, and the length of necrosis from the inoculation point to the margin of healthy tissue was measured on each stem at 2 wk after inoculation (.)
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