Differential responses of 23 maize cultivar seedlings to an arbuscular mycorrhizal fungus when grown in a metal-polluted soil

Abstract

Modern breeding efforts have been accelerating crop improvement and yielding numerous cultivars with distinct genetic traits; however, interactions between different cultivars and their root-associated arbuscular mycorrhizal fungi (AMF) are not clear. Herein, we selected the 22 most common commercial maize (Zea mays) varieties in China and an inbred line (B73) to study the differential responses of these 23 cultivars to mycorrhizal inoculation when grown in an arable soil polluted by multiple metals (Pb, Zn, and Cd). We found that the different cultivars exhibited significant variations in plant metal accumulation, ranging from strong metal exclusion (ZYY9) to strong metal accumulation (B73). Mycorrhizal colonization substantially altered metal uptake and repartitioning, while bioaugmenting the inherent characteristics of metal accumulation; for example, the AMF enhanced leaf accumulation of the metal-accumulator B73, and markedly reduced the root uptake of the metal-excluder ZYY9. However, such AMF-induced alterations were also substantially dependent on plant organs (roots and shoots) and metal species. We found that the extent of the AMF-induced leaf alterations was substantially greater than that of the root alterations. Similarly, the number of instances where the AMF significantly altered the Zn and Cd accumulation was far higher than the number of instances where Pb accumulation was significantly altered by AMF. In addition, the presence of AMF appeared to trigger the maize antioxidant systems, which may have alleviated the toxicity of excessive Cd, increased the leaf chlorophyll content, augmented the net photosynthetic rate, and promoted the growth of 17.39% of the maize cultivars. Our results suggest that a future crop breeding challenge is to produce cultivars for safe production or phytoremediation, thereby optimizing the combinations of crop cultivars and their root-associated AMF in slightly to moderately metal-polluted arable soils.