Abstract
Highly productive trans-resveratrol (t-R) grapevine suspension cultured cells (SCC) and two effective elicitors, methyl jasmonate (MJ) and randomly methylated β-cyclodextrins (CDs), were used to analyze the extent to which salt treatments alter the production of bioactive phenolic compounds. The expression/activity profile of the enzymes involved in phenol metabolism and antioxidant networks were also studied. A marked extracellular accumulation of phenolic compounds, especially t-R, was found in SCC elicited with CDs and/or MJ under saline conditions. However, the treatments with MJ alone and all those combined with salt favored the intracellular accumulation of catechin and ferulic acid. The salt-induced accumulation of phenolics was correlated with the higher total antioxidant capacity values found in cells, suggesting that cellular redox homeostasis under saline conditions was largely maintained by increasing phenolic compound production. These higher levels of phenolics found in elicited cells under saline conditions fit well with the highest activity of phenylalanine ammonia-lyase. Moreover, antioxidant enzyme activities were boosted in treatments with MJ and/or in those combined with salt and decreased in those treated with CDs. These results suggest a differential response of the antioxidative network to the presence of elicitors under saline conditions.
Highlights
Accepted: 12 February 2022 trans-Resveratrol (3,40,5-trihydroxystilbene; t-R) is a polyphenolic compound of a stilbene nature found in several dietary plants, such as plums, red berries, peanuts, or grapes, and some beverages, such as red wine [1,2]
This reduction in cell growth capacity was similar to suspension cultured cells (SCC) decreased to 50% (Figure 1a)
The presence of salt in combination with CDs or CDs + methyl jasmonate (MJ) led to a further decrease in cell biomass of salt in combination with CDs or CDs + MJ led to a further decrease−1in cell biomass
Summary
Accepted: 12 February 2022 trans-Resveratrol (3,40 ,5-trihydroxystilbene; t-R) is a polyphenolic compound of a stilbene nature found in several dietary plants, such as plums, red berries, peanuts, or grapes, and some beverages, such as red wine [1,2]. T-R is involved in the constitutive and inducible defense responses against both biotic and abiotic stresses [3,4]. T-R is well known for its relevant benefits on human health [5]. Its health-promoting effects are well-correlated with its cell defense properties [7]. T-R can directly act as a scavenger of reactive oxygen/nitrogen species, or can indirectly enhance the antioxidative pathways responsible for the maintenance of cellular redox homeostasis [7]. Due to its therapeutic potential, the demand for t-R keeps on rising for pharmaceutical, nutraceutical, and cosmetic uses [8]. An efficient system for the production of t-R consists in the use of plant cell cultures as biofactories under elicitation [9]
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