Differential response of Germplasm to Inter-Specific Hybridization of Brassica spp.

Abstract

Differential response of Germplasm to Inter-Specific Hybridization of Brassica spp. Understanding the fact behind the incompatible reaction in inter specific crosses is important for attempting successful crosses. Inter-specific hybridization between Brassica juncea and Brassica oleracea var botrytis were developed using reciprocal crosses. Four varieties of Indian mustard namely Varuna, Kranti, Pusa Bold and Rajendra Suflam were crossed reciprocally with one Cauliflower variety Sabour Agrim. Several barriers were observed right from the pre-fertilization to post fertilization during embryo developmental stages. 78.26%, 64.96% and 63.68% siliqua was retained after 6 days, 10 days and 15 days of pollination respectively when B. juncea was used as female parent whereas 99.35%, 45.51% and 43.22% of siliqua were retained after 6 days, 10 days and 15 days of pollination respectively when Brassica oleracea var bortytis was used as female parent. Both pre and post fertilization barriers were predominantly found when B. juncea was used as female parent whereas post fertilization barriers were found in the reciprocal crosses. Differential varietal response to siliqua drops was observed in the reciprocal crosses. Maximum response in the success of crosses was obtained for variety Kranti in B. juncea X B oleracea cross whereas variety Rajendra suflam was highly efficient in reciprocal crosses. ..