Abstract
In this work the activity of superoxide dismutase (SOD) and the enzymes of the ascorbate-glutathione (ASC-GSH) cycle were investigated in chloroplasts and mitochondria from leaves of Pisum sativum L. cv. Puget after 15 days treatment with 0-130 mM NaCl. The main chloroplastic SOD activity was due to CuZn-SOD II, which was increased significantly (about 1.7-fold) by NaCl, although during severe NaCl stress (110-130 mM) chloroplastic Fe-SOD exhibited a stronger enhancement in its activity (about 3.5-fold). A sudden induction in chloroplastic APX, DHAR and GR was also caused by NaCl (70-110 mM), but not by the highest salt concentration (130 mM), at which GR and DHAR activities were similar to the control values and APX decreased. In addition, the H2O2 concentration and lipid peroxidation of membranes increased significantly, 3.5- and 7-fold, respectively, in chloroplasts under severe NaCl stress. In purified mitochondria DHAR and GR were significantly induced only at 90 and 130 mM NaCl, respectively, although DHAR activity was below control values in the highest NaCl concentrations. APX and MDHAR activities started their response to salt in mild NaCl conditions (70 mM) and increased significantly with the severity of the stress. Mn-SOD was induced only under severe NaCl concentrations. The mitochondrial H2O2 and lipid peroxidation were increased at the highest NaCl concentration although to a lesser extent (about 2-2.5-fold) than in chloroplasts, whereas the increase in carbonyl protein contents was higher in mitochondria. The results suggest that the degree of enhanced tolerance to NaCl seems to require the induction of specific isoforms, depending on the different organelles.
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