Abstract
Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst. This study was to investigate the effect of asynchronous embryo transfer on uterine receptivity in mice. Embryos under different stages were transferred into two oviduct sides of a recipient mouse on day 1 of pseudopregnancy. Our results showed the asynchronously transferred embryos can implant in all groups. Compared to zygote-transfer group, the length of implanted embryos is longer in 8-cell embryo- or blastocyst-transfer group. The levels of Snail and COX-2 immunostaining in blastocyst-transfer group are significantly stronger than that in zygote-transfer group. Embryos in blastocyst-transfer group migrate faster than that in zygote-transfer group within uterus. Blastocysts are in a state of developmental delay after they are transferred into oviducts, and they are reactivated and implanted rapidly in uterus. The developmental rate to newborn in zygote-transfer group is obviously higher than that in blastocyst-transfer group, suggesting that a delay in embryo development and implantation will lead to a decrease of litter size. These results indicated that the window of implantation is differentially regulated in two uterine horns of a recipient by embryos at different stages.
Highlights
Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst
After asynchronous embryos are transferred into the genital tract in a recipient mouse, embryos at a later stage were considered to stop their development before the implantation window is ready
It is generally acknowledged that the establishment of uterine receptivity is mainly regulation by steroid hormones[1,20]
Summary
Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst. The developmental rate to newborn in zygotetransfer group is obviously higher than that in blastocyst-transfer group, suggesting that a delay in embryo development and implantation will lead to a decrease of litter size. These results indicated that the window of implantation is differentially regulated in two uterine horns of a recipient by embryos at different stages. Embryo implantation is a process that active blastocysts act with the uterus in receptive state and establish a close connection with uterine endometrium. Endometrial receptivity only lasts for a limited time In this period can the embryo make a connection with uterine endometrium. We are wondering whether the pregnancy outcome is different after zygotes or blastocysts were transferred into oviducts on day 1 of pseudopregnancy
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