Differential regulation of adipokine levels after Bariatric surgery (LB345)

Abstract

Bariatric surgery is known to cause significant weight loss, reduction in insulin resistance, cardiovascular disease and mortality. We hypothesized that adipose and systemic inflammation will be decreased post bariatric surgery. Our objective was to study changes in adipokine levels following bariatric surgery. We recruited patients undergoing Roux En Y gastric bypass and laparoscopic gastric band placement. Demographic data and both serum and adipose tissue samples were collected at the time of surgery, 2 weeks and 6 months post operatively. Luminex Human cytokine and adipokine kits were used along with adiponectin, Tumor Necrosis Factor‐α (TNF‐α), leptin and Monocyte Chemotractant Protein‐1 (MCP‐1) ELISAs to measure levels of these cytokines in serum and/or adipose tissues. Percentage changes in adipokines were calculated from time of surgery to each time point after surgery. There was a significant increase in adipose adiponectin at each post‐surgery time. Serum adiponectin showed an increasing trend by 6 months, but was not significant. Serum leptin, TNFα and MCP‐1 all showed decreasing trends from time of surgery to 6 months post surgery, but differences were not statistically significant. The percentage reduction for MCP‐1 at 6 months was significantly higher than for TNF‐α. These findings suggest that serum and adipose adipokine levels are differentially regulated by bariatric surgery. Additional studies are necessary to determine how these changes in tissue and circulating cytokine levels relate to various bariatric surgery outcomes.Grant Funding Source: PMERF (Physician’s Medical Education and Research Foundation, Knoxville, TN) and VPR and COHS Texas Tech University startup funds, Lubbock, TX