Abstract
The efficiency of leukocyte removal was studied by the removal of the buffy coat layer from fresh and overnight-stored whole blood. Manual blood processing was compared with two types of automated separators: the T-ACE (Terumo) and the OPTI system (with the top-and-bottom bag, Baxter). The main characteristics of leukocyte removal were similar for the systems studied. The efficiency of leukocyte removal was consistently higher with overnight-stored blood. When the net red cell content of the buffy coat was 20–30 mL, the figures were: 70% ( n=103) vs 45% ( n=87, manual method), 78% ( n=6) vs 60% ( n=100, T-ACE) and 82% ( n=10) vs 71% ( n=20, OPTI system), respectively. The increase in the efficiency in leukocyte removal was due to a rapid increase in the efficiency of removal of granulocytes: 65% ( n=65) vs 45% ( n=57, manual method), 80% ( n=4) vs 46% ( n=83, T-ACE) and 74% ( n=9) vs 57% ( n=17, OPTI system), respectively. Processing of overnight stored blood may provide red cell concentrates with fewer contaminating leukocytes and granulocytes.
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