Abstract
A procedure for determination of nanogram quantities of RNA in the presence of an excess of DNA and monomeric nucleic acid constituents is presented. The procedure is based on immobilization of RNA by incubation of a hanging mercury drop electrode in a 5-μL sample followed by washing and transfer of the nucleic acid-modified electrode in a voltammetric cell containing blank background electrolyte. The differential pulse voltammetric response of RNA differs from that of DNA. This makes it possible to determine traces (less than 1%) of RNA in DNA samples. Low molecular mass substances such as bases, nucleosides, and nucleotides do not interfere with the RNA determination as they do not adsorb at the electrode surface or are removed during the washing step. The limit of detection is below 100 pg of RNA
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