Abstract
The analytical application of differential pulse polarography (d.p.p) for the quantitative determination of cytosine, cytidine, cytidylyl-(3′–5′)-cytidine (CpC) and cytidylyl-(3′–5′)-adenosine (CpA) has been investigated. The dependence of the differential pulse peak on various quantities (pH, pulse amplitude, scan rate and drop time) was studied and the optimum conditions for the analytical determination of these compounds were found. The detection limit for cytidine, CpC and CpA was 0.5–1.0 μ M; for cytosine it was 5 μ M. Results of the analysis of mixtures of cytidine-cytosine, cytidine-CpA and cytidine-CpC are reported under the optimum conditions. The analytical determination of cytidine, CpC and CpA in the presence of the same concentration of cytosine was also investigated.
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