Abstract
The electrochemical behavior of chlorzoxazone at the carbon paste electrode was investigated in 0.04 mol/L Britton-Robinson buffer pH 6.50 using cyclic and differential pulse voltammetric techniques. Cyclic voltammetric studies indicated that the oxidation of the drug was irreversible and controlled mainly by diffusion. Experimental and instrumental parameters were optimized (50 mV/s scan rate, 50 mV pulse amplitude, and 0.04 mol/L Britton-Robinson (BR) buffer pH 6.50 as a supporting electrolyte) and a sensitive differential pulse anodic voltammetric method has been developed for the determination of the drug over the concentration range 0.17-1.68 µg/mL chlorzoxazone, with detection and quantitation limits of 0.05 and 0.16 µg/mL, respectively. The proposed voltammetric method was successfully applied to the determination of the drug in its pharmaceutical formulation (Myoflex tablets), and in spiked human urine samples.
Highlights
Chlorzoxazone, 5-chloro-2-hydroxy benzoxazole [95-25-0] (Scheme 1), is a centrally acting skeletal muscle relaxant with sedative properties
There is no published work concerning the anodic voltammetric determination of chlorzoxazone using the carbon paste electrode and in continuation of our previous work,[40,41,42,43] in this work, the electrochemical behavior of chlorzoxazone at carbon paste electrode was investigated, and a differential pulse anodic voltammetric method was developed for the determination of this drug
0.0170 g of chlorzoxazone was dissolved in methanol and introduced into 100 mL volumetric flask, 5 mL of urine from a healthy person was added, and the mixture was completed to the mark by methanol to prepare 10–3 mol/L chlorzoxazone in the spiked human urine sample. 10 mL of 0.04 mol/L BR buffer pH 6.5 was introduced into the voltammetric cell, different amounts of the above spiked human urine sample were added and the procedure was repeated as described before
Summary
Chlorzoxazone, 5-chloro-2-hydroxy benzoxazole [95-25-0] (Scheme 1), is a centrally acting skeletal muscle relaxant with sedative properties. It is claimed to inhibit muscle spasm by exerting an effect primarily at the level of the spinal cord and subcortical area of the brain. Various analytical methods have been reported in the literature for the determination of chlorzoxazone These include high performance liquid chromatography,[3,4,5,6,7,8,9,10,11,12,13] thin layer chromatography,[14,15,16,17] liquid chromatography-tandem mass spectrometry,[18] packed column supercritical fluid chromatography,[19,20] gas chromatography,[21,22,23] spectrophotometry,[24,25,26,27,28,29,30,31,32,33,34,35] fluorimetry,[36] and capillary zone electrophoresis.[37] Two papers have been described in literature concerning the voltammetric determination of chlorzoxazone based on the oxidation of the drug at the glassy carbon electrode and gold electrode.[38,39] The carbon paste electrodes have been extensively used in electroanalytical methods due to their excellent properties, like, wide potential range, low background current, easy surface renewal, easy preparation, and low cost.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
