Abstract
Global warming increases sea temperature and leads to high temperature stress, which affects the yield and quality of Pyropia haitanensis. To understand the molecular mechanisms underlying high temperature stress in a high temperature tolerance strain Z-61, the iTRAQ technique was employed to reveal the global proteomic response of Z-61 under different durations of high temperature stress. We identified 151 differentially expressed proteins and classified them into 11 functional categories. The 4 major categories of these are protein synthesis and degradation, photosynthesis, defense response, and energy and carbohydrate metabolism. These findings indicated that photosynthesis, protein synthesis, and secondary metabolism are inhibited by heat to limit damage to a repairable level. As time progresses, misfolded proteins and ROS accumulate and lead to the up-regulation of molecular chaperones, proteases, and antioxidant systems. Furthermore, to cope with cells injured by heat, PCD works to remove them. Additionally, sulfur assimilation and cytoskeletons play essential roles in maintaining cellular and redox homeostasis. These processes are based on signal transduction in the phosphoinositide pathway and multiple ways to supply energy. Conclusively, Z-61 establishes a new steady-state balance of metabolic processes and survives under higher temperature stress.
Highlights
The mechanisms of responding to high temperature stress have been investigated in a great deal of higher plants, especially in model plants and economic crops, and various mechanisms have been summarized
Since changes in transcription often do not correspond with changes in protein expression[13], comparative proteomic analysis based on 2-dimensional electrophoresis (2-DE) was conducted to screen the differentially expressed proteins in Z-61 under normal temperature and high temperature stress
To assess the reproducibility of the isobaric tags for relative and absolute quantitation (iTRAQ) data, Principal component analysis (PCA) was performed on every 2 replicates at each time point (Fig. 1)
Summary
The mechanisms of responding to high temperature stress have been investigated in a great deal of higher plants, especially in model plants and economic crops, and various mechanisms have been summarized. High throughput experimental methods such as transcriptomics and proteomics were utilized to identify stress-responsive genes and proteins regulated by elevated temperatures in Pyropia. ITRAQ is the most popular technique in plant quantitative proteomics[16,17] This technique is based on tagging the N-terminus of peptides generated from tryptic protein digests, which overcomes some limitations of gel-based techniques and improves the throughput of proteomic studies. This technique has a high degree of sensitivity, and the amine specific isobaric reagents permit the identification and quantitation of up to 8 different samples simultaneously[16,17]. In this paper, we employed iTRAQ and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to further understand the molecular mechanisms underlying high temperature stress in Pyropia and reveal the global proteomic response of the Z-61 stains of P. haitanensis under high temperature stress
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
