Differential proteomes of the cyanobacterium Cyanothece sp. CCY 0110 upon exposure to heavy metals.

Rita Mota,Marianna Meazzini,Rui Fernandes,Sara B Pereira,Paula Tamagnini,Phillip C Wright,Roberto De Philippis,Caroline A Evans,Arlete Santos

doi:10.1016/j.dib.2015.04.015

Abstract

The proteomes of the highly efficient extracellular polymeric substances (EPS)-producer cyanobacterium Cyanothece sp. CCY 0110, grown in medium supplemented with an essential metal (Cu2+) or a non-essential metal (Cd2+),were compared using iTRAQ technology. The data were obtained within a larger study that evaluated the overall effects of different heavy metals on growth/survival, EPS production and ultrastructure of this cyanobacterium [1]. To allow a broader understanding of the strategies triggered to coupe with toxic effects of the metals, Cyanothece′s proteomes were evaluated after chronic and acute exposure to Cu2+ and Cd2+ in two independent 8-plex iTRAQ studies. For the chronic exposure 0.1 mg/l of Cu2+ or 5 mg/l of Cd2+ were used for 10 and 20 days, while in the acute experiments the cells were exposed to 10× these concentrations for 24 h. 202 and 268 proteins were identified and quantified for studies 1 (Cu2+) and 2 (Cd2+), respectively. The majority of the proteins with significant fold changes were associated with photosynthesis, CO2 fixation and carbohydrate metabolism, translation, and nitrogen and amino acid metabolism.

Highlights

The proteomes of the highly efficient extracellular polymeric substances (EPS)-producer cyanobacterium Cyanothece sp
CCY 0110, grown in medium supplemented with an essential metal (Cu2 þ) or a non-essential metal (Cd2 þ),were compared using isobaric tags for relative and absolute quantification (iTRAQ) technology
To allow a broader understanding of the strategies triggered to coupe with toxic effects of the metals, Cyanothece0s proteomes were evaluated after chronic and acute exposure to Cu2 þ and Cd2 þ in two independent 8-plex iTRAQ studies

Summary

Organism and culture conditions

CCY 0110 (Culture Collection of Yerseke, The Netherlands) was grown in 100 ml Erlenmeyer flasks containing ASNIII medium [2] (control) or in medium supplemented with 0.1 or 1 mg/l of copper (Cu2 þ, stock solution 10,000 mg/l in 1% HNO3, Sigma-Aldrich Co., MO, USA), or 5 or 50 mg/l of cadmium (Cd2 þ, stock solution 10,000 mg/l in 5% HNO3, Sigma-Aldrich). The biological replicates used as control were common to the two studies. Both studies comprised four phenotypes of cells grown:. (C1, C2) in ASNIII buffered medium for 10 days (control). (Cu3, Cu4 and Cd3, Cd4) in medium supplemented with either 0.1 mg/l of Cu2 þ or 5 mg/l of Cd2 þ for 20 days (chronic exposure). (C1, C2) in ASNIII buffered medium for 10 days (control). (Cu1, Cu2 and Cd1, Cd2) in medium supplemented with either 0.1 mg/l of Cu2 þ or 5 mg/l of Cd2 þ for 10 days (chronic exposure). (Cu3, Cu4 and Cd3, Cd4) in medium supplemented with either 0.1 mg/l of Cu2 þ or 5 mg/l of Cd2 þ for 20 days (chronic exposure). (Cu5, Cu6 and Cd5, Cd6) in medium supplemented with either 1 mg/l Cu2 þ or 50 mg/l Cd2 þ for 24 h (acute exposure)

Protein extraction and quantification

MS data analysis

Findings

Statistical analysis