Abstract
Abstract 4845To screen molecular markers for refractory anemia with excess blasts in transformation (RAEB) in myelodysplastic syndromes (MDS) by serum proteome profiling. MethodsSerum protein were isolated respectively from patients with RAEB, acute myeloid leukemia or normal person by 2-dimensional electrophoresis(2-DE). The imaging films were obtained and the differential reacting protein spots were recognized. Then the differential reacting proteins were obtained in the replica gel by matching analysis,and identified by peptide mass fingerprint based on matrix-assisted laser desorption/ionization time of-flight mass spectrometry (MALDI-TOF-MS) and database searching. ResultsSeven differentially expressed proteins in RAEB were found by 2-DE. Of the 7 proteins, 4 were identified with significantly statistical difference, including dipeptidyl peptidase (DPP/CD26), polymerase (DNA directed) kappa, PRO2044 and albumin-like. Conclusion2-DE-based serum proteome profile can help to identify serum proteomic biomarkers related to MDS. DDP/CD26 has increased serum levels in RAEB subtype MDS, suggesting significant action in advanced MDS. DisclosuresNo relevant conflicts of interest to declare.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
