Abstract
Better knowledge on responses to dehydration stress could help to improve the existing cryopreservation protocols for potato, since plant tissues processed for cryopreservation are often submitted to similar in vitro stress conditions. Cryopreservation (the best method of conservation for vegetatively propagated plants) of potato still needs to be standardized to make it available and to conserve the wide diversity of this crop. In the present work, the response to osmotic stress and chilling temperature was investigated in two potato species, Solanum tuberosum and its relative, frost-tolerant S. commersonii. After 14 days of exposure, different growth parameters, such as shoot length and number of leaves, were measured. Furthermore, differentially abundant proteins were identified after performing 2-fluorescence difference gel electrophoresis (2-DIGE) experiments, and soluble carbohydrates were analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). The results show different responses in both species depending on the stress treatment. Focusing on the differences in growth parameters during the treatments, Solanum commersonii seems to be more affected than S. tuberosum cv. Désirée. At the molecular level, there are some differences and similarities between the two potato species studied that are dependent on the type of stressor.
Highlights
Potato (Solanum spp.) is one of the most cultivated species on earth and ranks fifth in production [1]
Our results indicate that plastid-specific protein synthesis is inhibited, and degradation is facilitated under osmotic stress condition in S. commersonii and suggests a more pronounced stress-related response in this species, as compared to Désirée
We have identified a heat-shock protein 70 (Hsp70) that increases its quantity in S. commersonii after exposure to chilling temperature
Summary
Potato (Solanum spp.) is one of the most cultivated species on earth and ranks fifth in production [1]. Environmental constraints, like drought or low temperatures, can have adverse effects on plants and, as a consequence, can induce stress responses at the molecular and physiological level [4] that often leads to yield loss. If such exposure is extended in time, stress causes major alterations in the plant metabolism that leads to cell death [5]. To our knowledge, this is the first time that this type of combined study (proteomic and targeted metabolites analyses) compares S. tuberosum with its frost-tolerant relative, S. commersonii, to highlight some common and different mechanisms in response to abiotic stress
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