Abstract
We used proteomic approaches to study the survival and defense mechanisms of Antarctic microalga under heat stress. The microalga was cultured for optimal growth at 4°C, the temperature was increased to 15°C, and the resultant differentially expressed proteins induced by heat stress were analyzed by mass spectrometry following two-dimensional gel electrophoresis. Orthologues of 22 proteins showed more than two-fold changes in abundance; of these, 8 proteins were up-regulated, and 14 were down-regulated. In addition, changes in the enzyme activities and isozyme profiles of catalase, aldehyde dehydrogenase, superoxide dismutase, glutathione reductase, and ascorbate peroxidase were investigated using an in-gel activitystaining method. Alterations in protein expression and antioxidant enzyme activity in Antarctic algae may be related to survival and defense mechanisms against elevated temperatures.
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