Differential projections to the raphe nuclei from the medial parabrachial-Kölliker-Fuse (NPBM-KF) nuclear complex and the retrofacial nucleus in cats: retrograde WGA-HRP tracing

Abstract

Abstract After injection of wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP, 2 × 30 nl) into the nucleus raphe magnus (NRM) of 6 cats, a number of retrogradely labelled neurons were observed in the rostral pons, mainly in the pontine pneumotaxic area, i.e., the medial parabrachial and Kolliker-Fuse (NPBM-KF) nuclear complex, and the tegmental field. In addition, a cluster of labelled cells was observed in the retrofacial nucleus (RFN) and its adjacent areas in the medulla. Control injections of the same volume of WGA-HRP into the medullary magnocellular tegmental field (2 mm lateral to the NRM) resulted in a much lower number of labelled neurons in the areas described above, and the labelled cells in the tegmental fields were predominant ipsilateral to the injected side. Injections into the nucleus raphe pallidus caudal to the NRM resulted in a diffuse distribution of labelled neurons, mainly in the tegmental fields of the pons and medulla. This study demonstrates that the NRM receives specific convergent projections from the NPBM-KF complex and the RFN in the medulla. It is suggested that these pathways are involved in the control of respiration.