Abstract
Differential Processing of Cytosolic and Mitochondrial Caspases.
Highlights
The mitochondria have been shown to play an important role in the initiation of caspase activation during apoptosis
Staurosporine treatment resulted in caspase-3 cleavage observed at 4 hours in both cytosol and mitochondria and with approximately similar kinetics
Caspase-9 processing in mitochondria indicated that the cytochrome c-dependent 35 kDa and caspase-3dependent 37 kDa forms were approximately equal in abundance while the 37 kDa form predominated in cytosol
Summary
The mitochondria have been shown to play an important role in the initiation of caspase activation during apoptosis. In Jurkat, CEM/Neo and CEM/Bcl-2 cell lines, apoptosis was induced with antiFas antibody (clone CH-11, Kamiya Biomedical Co.) or staurosporine (Sigma). Mitochondria were prepared from cells after disruption by nitrogen cavitation (4). Our studies indicated that caspases-3 and -9 were associated with the washed mitochondrial pellet as well as in the cytosol.
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