Abstract
Aqueous two phase extraction was employed as an alternate method for the downstream processing of anthocyanins. Polyethylene glycol (PEG) 4000/magnesium sulphate (14.8%/10.3% w/w) was found to be the most suitable system. Tie line length of 32.61% and volume ratio of 0.73 have resulted in maximum partitioning of anthocyanins to the PEG-rich phase (yield – 98.19%) and sugars to the salt-rich phase (yield – 73.16%). Multistage aqueous two phase extraction resulted in an increase in removal of sugars (to about 96.5%) with 91.9% yield of anthocyanins. The phase forming polymer (PEG 4000) was successfully separated from anthocyanins employing organic–aqueous extraction resulting in 2.3 fold increase in the concentration of anthocyanins. High performance liquid chromatography (HPLC)–Mass spectroscopy (MS)/MS was carried out to confirm the structural stability of anthocyanins.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
