Differential Nutrient Limitation of Soil Microbial Biomass and Metabolic Quotients (qCO2): Is There a Biological Stoichiometry of Soil Microbes?

Wyatt H Hartman,Curtis J Richardson,Melanie R Mormile

doi:10.1371/journal.pone.0057127

Abstract

BackgroundVariation in microbial metabolism poses one of the greatest current uncertainties in models of global carbon cycling, and is particularly poorly understood in soils. Biological Stoichiometry theory describes biochemical mechanisms linking metabolic rates with variation in the elemental composition of cells and organisms, and has been widely observed in animals, plants, and plankton. However, this theory has not been widely tested in microbes, which are considered to have fixed ratios of major elements in soils.Methodology/Principal FindingsTo determine whether Biological Stoichiometry underlies patterns of soil microbial metabolism, we compiled published data on microbial biomass carbon (C), nitrogen (N), and phosphorus (P) pools in soils spanning the global range of climate, vegetation, and land use types. We compared element ratios in microbial biomass pools to the metabolic quotient qCO2 (respiration per unit biomass), where soil C mineralization was simultaneously measured in controlled incubations. Although microbial C, N, and P stoichiometry appeared to follow somewhat constrained allometric relationships at the global scale, we found significant variation in the C∶N∶P ratios of soil microbes across land use and habitat types, and size-dependent scaling of microbial C∶N and C∶P (but not N∶P) ratios. Microbial stoichiometry and metabolic quotients were also weakly correlated as suggested by Biological Stoichiometry theory. Importantly, we found that while soil microbial biomass appeared constrained by soil N availability, microbial metabolic rates (qCO2) were most strongly associated with inorganic P availability.Conclusions/SignificanceOur findings appear consistent with the model of cellular metabolism described by Biological Stoichiometry theory, where biomass is limited by N needed to build proteins, but rates of protein synthesis are limited by the high P demands of ribosomes. Incorporation of these physiological processes may improve models of carbon cycling and understanding of the effects of nutrient availability on soil C turnover across terrestrial and wetland habitats.

Highlights

Variation in heterotrophic microbial metabolism poses a critical uncertainty in our current understanding of soil carbon (C) cycling in terrestrial and wetland soils, and improved understanding of microbial mediation of soil C and nutrient cycling is needed to predict ecosystem responses to human alteration of land use, climate, and nutrient availability [1,2,3,4]
Global variation in the stoichiometry of soil resources a previous study of forest and grasslands suggested that pools of soil C, N, and P are closely linked with allometric relationships describing nearly fixed stoichiometry [10], our broader data set indicated considerable differences in the C:N:P stoichiometry of terrestrial and wetland soils, especially with respect to the relative availability of P
Our results directly showed that the C:N:P stoichiometry of soil microbes does not occur in strictly fixed isometric ratios

Summary

Introduction

Variation in heterotrophic microbial metabolism poses a critical uncertainty in our current understanding of soil carbon (C) cycling in terrestrial and wetland soils, and improved understanding of microbial mediation of soil C and nutrient cycling is needed to predict ecosystem responses to human alteration of land use, climate, and nutrient availability [1,2,3,4]. Our cross-ecosystem dataset included measurements of soil and microbial C, N, and P pools, and C mineralization rates in soils spanning global variation soil conditions and nutrient availability in terrestrial and wetland ecosystems, and we explicitly considered study location factors of climate, land use, and vegetation as predictor variables.

Results

Conclusion