Abstract
Previous studies have characterized NAD(P)H oxidases (NOX) in bovine pulmonary and coronary arteries in the context of examining their differential role in responses associated with changes in PO2. In the present study we compared NOX expression and activity in rat intrapulmonary (RPA) and coronary (RCA) arteries. In RPA there was an elevated production of NOX‐derived O2.‐ and H2O2, as compared to RCA. Microarray gene expression profiling revealed that among the factors known to influence NOX activity, gp91phox, glucose‐6‐phosphate dehydrogenase (G6PD) and bone morphogenetic protein‐4 (BMP4) were enriched in RPA, as compared to RCA. Protein expression of gp91phox in RPA was also increased. BMP4 (3 ng/mL) elicited significant NOX‐dependent increases in ROS production in RCA and human CAECs (∼250%). However, BMP4 ‐induced increases in ROS generation in RPA and PAECs (∼120%) were significantly lower. Incubation with an anti‐BMP4 antibody did not decrease ROS generation significantly in RPA. Thus, both rat and bovine PAs exhibit increased NOX activity, likely due to an increased expression/activity of gp91phox and G6PD, whereas our data does not support a major role for BMP4 in this phenomenon.
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