Differential mucin expression by respiratory syncytial virus and human metapneumovirus infection in human epithelial cells.

Ma Del Rocío Baños-Lara,Boyang Piao,Antonieta Guerrero-Plata

doi:10.1155/2015/347292

Ma Del Rocío Baños-Lara, Boyang Piao + Show 1 more

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https://doi.org/10.1155/2015/347292

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Journal: Mediators of inflammation	Publication Date: Jan 1, 2015
Citations: 60	License type: CC BY 3.0

Affiliation: Louisiana State University

Abstract

Mucins (MUC) constitute an important component of the inflammatory and innate immune response. However, the expression of these molecules by respiratory viral infections is still largely unknown. Respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) are two close-related paramyxoviruses that can cause severe low respiratory tract disease in infants and young children worldwide. Currently, there is not vaccine available for neither virus. In this work, we explored the differential expression of MUC by RSV and hMPV in human epithelial cells. Our data indicate that the MUC expression by RSV and hMPV differs significantly, as we observed a stronger induction of MUC8, MUC15, MUC20, MUC21, and MUC22 by RSV infection while the expression of MUC1, MUC2, and MUC5B was dominated by the infection with hMPV. These results may contribute to the different immune response induced by these two respiratory viruses.

Highlights

The mucosal surface of the respiratory tract is protected by a layer of mucus [1], a viscoelastic, gel-like substance that covers the epithelial surface of various mammalian tissues, including the respiratory tract
In this work we investigated the MUC response to Respiratory syncytial virus (RSV) and human metapneumovirus (hMPV), two closest related human paramyxoviruses, which despite their shared structural and epidemiological characteristics differ in many aspects of the immune response they trigger in the infected host
Knowledge of the host immune response induced by RSV and hMPV is critical to understanding the viral pathogenesis of these highly relevant respiratory viruses

Summary

Introduction

The mucosal surface of the respiratory tract is protected by a layer of mucus [1], a viscoelastic, gel-like substance that covers the epithelial surface of various mammalian tissues, including the respiratory tract. The main functions of mucus include protecting the epithelial surface from injury, by facilitating removal of materials that enter the lung and in the pathogenesis of many lung diseases, those involving chronic inflammation of the airways or susceptibility to infection. MUC, which have a transmembrane domain that anchors them to the cell membrane. The members in this group are MUC1, MUC3A/B, MUC4, MUC11, MUC12, MUC13, MUC15, MUC16, MUC17, MUC18, MUC20, MUC21, and MUC22 and secreted mucins: MUC2, MUC5AC, MUC5B, MUC6, MUC7, MUC8, and MUC19. MUC9 is both located in the cell surface and secreted as well. Overexpression of MUC has been reported in several malignancies like breast, gastric, colorectal, pancreatic, lung, small bowel, and ovarian cancers [6, 7]

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