Differential modulation of courtship behavior and subsequent aggression by octopamine, dopamine and serotonin in male crickets

Abstract

Aggression is a behavioral strategy for securing limited resources and its expression is strongly influenced by their presence and value. In particular, males are generally thought to guard females after mating to ward off other males, but the underlying control mechanisms are unknown. Here, we investigated the role of amines on male courtship behavior and its subsequent effect on male-male aggression in crickets (Gryllus bimaculatus). Contrary to the guarding hypothesis, female presence alone had no immediate effect on male-male aggression. Furthermore, confirming studies on other species, prior female contact, but not necessarily courtship or copulation, promoted subsequent male-male aggression in subordinate, but not socially naive crickets. This promoting effect of female contact is transient and slowly wanes after her removal. Selective aminergic receptor antagonists revealed that the promoting effect of prior female contact on male-male aggression is mediated by octopamine (OA), as well as by serotonin (5HT) acting most likely via 5HT1 and/or 5HT7 like receptors. This contrasts the role of 5HT2-like receptors in maintaining reduced aggressiveness after social defeat. Furthermore, while dopamine (DA) is necessary for the recovery of aggression in subordinates after defeat, it appears to play no part in female induced aggression. Male courtship, on the other hand, is selectively promoted by DA and 5HT, again most likely via 5HT1 and/or 5HT7 like receptors, but not by OA. We conclude that OA, DA and 5HT each differentially modulate different aspects of courtship and aggressive behavior in a context specific fashion.