Abstract
BackgroundMicroRNAs play an important role in many fundamental biological and pathological processes. Defining the microRNAs profile underlying the processes by beneficial and detrimental lifestyles, including caloric restriction (CR), exercise and high-fat diet (HF), is necessary for understanding both normal physiology and the pathogenesis of metabolic disease. We used the microarray to detect microRNAs expression in livers from CR, EX and HF mice models. After predicted potential target genes of differentially expressed microRNAs with four algorithms, we applied GO and KEGG to analyze the function of predicted microRNA targets.ResultsWe describe the overall microRNAs expression pattern, and identified 84 differentially expressed microRNAs changed by one or two or even all the three lifestyle modifications. The common and different enriched categories of gene function and main biochemical and signal transduction pathways were presented.ConclusionsWe provided for the first time a comprehensive and thorough comparison of microRNAs expression profiles in liver among these lifestyle modifications. With this knowledge, our findings provide us with an overall vision of microRNAs in the molecular impact of lifestyle on health as well as useful clues for future and thorough research of the role of microRNAs.
Highlights
MicroRNAs play an important role in many fundamental biological and pathological processes
Our findings provide us with an overall vision of microRNAs in the molecular impact of lifestyle on health as well as useful clues for future and thorough research of the role of microRNAs
In conclusion, this study provided for the first time a comprehensive and thorough comparison of microRNAs expression profiles in liver among beneficial and detrimental lifestyles, including caloric restriction (CR), EX and high-fat diet (HF)
Summary
MicroRNAs play an important role in many fundamental biological and pathological processes. Defining the microRNAs profile underlying the processes by beneficial and detrimental lifestyles, including caloric restriction (CR), exercise and high-fat diet (HF), is necessary for understanding both normal physiology and the pathogenesis of metabolic disease. MicroRNAs are a class of short non-coding RNAs which primarily interact with 3′ untranslated region (3’UTR) of mRNA, leading to either translational repression or mRNA degradation [7] These small molecules regulate approximate one third of the protein-coding genes, directly or indirectly involve in almost all cellular pathways [8]. Defining the microRNAs profile underlying the control of hepatic functions and processes by CR, EX and HF is necessary for understanding both normal physiology and the pathogenesis of metabolic disease
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
