Abstract
BackgroundPrimary Sjögren’s syndrome (pSS) is a chronic autoimmune disease characterized by abnormal immune cell activation. This study aimed to investigate differentially expressed long non-coding RNA (lncRNA) in peripheral blood mononuclear cells (PBMCs) in patients with pSS to identify lncRNAs that affect pSS pathogenesis.MethodsTotal RNA was extrated from PBMCs of 30 patients with pSS and 15 healthy persons. Transcriptome sequencing was used to screen differentially expressed lncRNAs and mRNAs in 8 RNA samples from the discovery cohort. The differentially expressed mRNAs underwent functional enrichment analysis. A protein interaction relationship (PPI) and competitive endogenous RNA (ceRNA) network was constructed. Real-time PCR was used to validate screened lncRNAs in all 45 RNA samples..Results1180 lncRNAs and 640 mRNAs were differentially expressed in pSS patients (fold change > 2 in healthy persons). The PPI network was constructed with 640 mRNAs and a ceRNA network with four key lncRNAs (GABPB1-AS1, PSMA3-AS1, LINC00847 and SNHG1). Real-time PCR revealed that GABPB1-AS1 and PSMA3-AS1 were significantly up-regulated 3.0- and 1.4-fold in the pSS group, respectively. The GABPB1-AS1 expression level was positively correlated with the percentage of B cells and IgG levels.ConclusionsGABPB1-AS1 was significently up-regulated in pSS patients, and its expression level is positively correlated with the percentage of B cells and IgG levels. GABPB1-AS1 may be involved in the pathogenesis of pSS and may be a promising biological marker.
Highlights
Primary Sjögren’s syndrome is a chronic autoimmune disease characterized by abnormal immune cell activation
High-throughput long non-coding RNA (lncRNA) and mRNA expression profile in Peripheral blood mononuclear cell (PBMC) We found 1180 lncRNAs and 640 mRNAs with significantly different expression in the PBMC of Primary Sjögren’s syndrome (pSS) patients based on transcriptome sequencing
We found that the expression level of GABPB1-AS1 is positively correlated with the percentage of B cells and IgG levels (Fig. 5b)
Summary
Primary Sjögren’s syndrome (pSS) is a chronic autoimmune disease characterized by abnormal immune cell activation. Large amounts of activated B lymphocytes are observed, which produce a variety of autoantibodies and cytokines; this is related to the occurrence and development of the disease. Multiple factors such as genetic and environmental factors are thought to be related to this abnormal cellular activity, the specific mechanisms of pSS have not been fully elucidated. A large number of studies have shown that miRNAs participate in regulating the pathogenesis of some autoimmune diseases due to their control of gene expression [1, 2].
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