Abstract
Differential interference contrast (DIC) microscopy is a technique to image spatially dependent gradients in optical path lengths. Contrast is produced through the splitting of polarized light with quartz Wollaston prisms. Here we demonstrate that light splitting for DIC microscopy can also be achieved with Sanderson prisms consisting of polycarbonate bars under a bending load. Comparable image contrast while imaging cultured cells was achieved with this alternative technique. These results demonstrate an inexpensive and easily adjustable alternative to traditional quartz Wollaston prisms.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
