Abstract
The effect of 7-methylguanosine 5′-monophosphate (m 7G 5′ p) on translation of partially purified globin mRNA and of polysome-associated endogenous globin mRNA has been studied. Under identical experimental conditions, with 0.4 mM m 7G 5′ p, translation with partially purified globin mRNA is inhibited 50%; translation with endogenous globin mRNA is inhibited 10%. The inhibition of protein synthesis by m 7G 5′ p occurs at a step before the first peptide bond formation as evidenced by studies with pactamycin; 0.4 mM m 7G 5′ p inhibited the first dipeptide synthesis 43% when the partially purified globin mRNA was used whereas 15% inhibition was observed with the endogenous mRNA. The inhibition of m 7G 5′ p appears to be related to the structural integrity of globin mRNA.
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