Differential Inhibition by Oxygen Radicals of Vasoactive Amines-Induced Contractions in the Porcine Coronary Artery

Abstract

Reactive oxygen species (ROS) play an important role in normal metabolic and signaling processes. Excess ROS, however, can cause severe cardiovascular damage. Thus, the present study was designed to examine effects of H(2)O(2) and xanthine plus xanthin oxidase (X/XO) on the serotonin (5HT), histamine (His) and acetylcholine (ACh)-induced contractions of porcine coronary arteries. In addition, to explore the site of ROS formation and species of it, the inhibitory effects of edaravone and EDTA were also tested. 5HT- and His-induced contractions were suppressed by H(2)O(2) and X/XO treatment. However, these suppressions of ACh-induced contraction by H(2)O(2) treatment was relatively weak and X/XO treatment caused no suppression on ACh-induced contraction. In the presence of edaravone which is thought to be a scavenger for .OH, significant decrease of inhibition of 5HT- and His-induced contractions was observed when coronary artery strips were treated with X/XO, but not H(2)O(2). On the other hand, inhibitory effects by EDTA treatments were also observed in X/XO treatments. These results suggest that 1) ROSs produced by additions of H(2)O(2) or X/XO are considered to be responsible for several physiological functions of coronary artery contractions, 2) the site of ROS produced by X/XO system, probably .OH, was outside the cell, but the inhibitory action of H(2)O(2), was inside the cell, and 3) a low susceptibility of ACh-induced contraction to H(2)O(2) and X/XO may indicates the signal transduction pathway(s) of ACh-induced contraction is different from those of 5HT and His.