Abstract
The effect of stimulating factors on the activity of aminoacyl- tRNA synthetases from bean leaves and Euglena gracilis cytoplasm and chloroplasts in the reaction of aminoacylation was investigated. Leucyl- tRNA synthetase was chosen as a model system. Stimulating factors isolated from bean, lupin and maize seeds have different effects on the activity of leucyl- tRNA synthetase. In all cases the stimulating factor from maize appeared to be active. The stimulating factors I and II from bean in a homologous system stimulate only the cytoplasmic enzyme. The most interesting systems were taken for further examination of the 32PP i-ATP exchange reaction, where a weaker effect was found.
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