Differential induction of adaptive responses by paraquat and hydrogen peroxide against the genotoxicity of methyl mercuric chloride, maleic hydrazide and ethyl methane sulfonate in plant cells in vivo

Abstract

Induction of adaptive response by conditioning doses of paraquat (PQ) and hydrogen peroxide (H 2O 2) in embryonic shoot cells of Hordeum vulgare and root meristem cells of Allium cepa was tested against the genotoxicity of challenge doses of methyl mercuric chloride (MMCl), maleic hydrazide (MH) or ethylmethane sulfonate (EMS). Plant tissue fixed at different recovery hours following the challenge treatments was analysed for cells with genotoxicity markers that include spindle or chromosome aberrations and micronuclei. The results provided clear-cut evidence that whereas H 2O 2 induced adaptive response for the chromosome damage caused by MMCl and MH, PQ induced the same for MMCl and EMS, but not for damage caused by MH. The findings pointed to the differences in the underlying mechanisms of oxidative responses induced by H 2O 2 and O 2 − · .