Abstract
BackgroundHost immunity plays an important role against oral microorganisms in periodontitis.MethodsThis study assessed the infiltrating immune cell subtypes in 133 healthy periodontal and 210 chronic periodontitis tissues from Gene Expression Omnibus (GEO) datasets using the CIBERSORT gene signature files.ResultsPlasma cells, naive B cells and neutrophils were all elevated in periodontitis tissues, when compared to those in healthy controls. In contrast, memory B cells, resting dendritic, mast cells and CD4 memory cells, as well as activated mast cells, M1 and M2 macrophages, and follicular helper T cells, were mainly present in healthy periodontal tissues. Furthermore, these periodontitis tissues generally contained a higher proportion of activated CD4 memory T cells, while the other subtypes of T cells, including resting CD4 memory T cells, CD8 T cells, follicular helper T cells (TFH) and regulatory T cells (Tregs), were relatively lower in periodontitis tissues, when compared to healthy tissues. The ratio of dendritic and mast cells and macrophages was lower in periodontitis tissues, when compared to healthy tissues. In addition, there was a significant negative association of plasma cells with most of the other immune cells, such as plasma cells vs. memory B cells (γ = − 0.84), plasma cells vs. resting dendritic cells (γ = − 0.64), plasma cells vs. resting CD4 memory T cells (γ = 0.50), plasma cells versus activated dendritic cells (γ = − 0.46), plasma cells versus TFH (γ = − 0.46), plasma cells versus macrophage M2 cells (γ = − 0.43), or plasma cells versus macrophage M1 cells (γ = − 0.40), between healthy control and periodontitis tissues.ConclusionPlasma cells, naive B cells and neutrophils were all elevated in periodontitis tissues. The infiltration of different immune cell subtypes in the periodontitis site could lead the host immunity against periodontitis.
Highlights
Host immunity plays an important role against oral microorganisms in periodontitis
Baseline characteristics of the datasets The present study obtained and analyzed the differentially expressed gene profiles between human healthy periodontal tissues (n = 133) and periodontitis tissues (n = 210), and applied the CIBERSORT gene signature file LM22 [16] to assess the different immune cell proportions in 133 healthy human periodontal tissues and 210 chronic periodontitis tissues. These tissue samples were taken from 210 non-smokers with periodontitis, and each contributed to a ≥ 2 “diseased” interproximal papillae, or “healthy” papilla, when such data was available (Table 1)
The plasma and naive B cells and neutrophils were all elevated in the periodontitis group, when compared to those for healthy controls (Fig. 1), while memory B cells, resting dendritic, mast and CD4 memory cells, as well as activated mast cells, M1 and M2 macrophages, and follicular helper T cells, were higher in healthy periodontal tissues versus periodontitis tissues (Fig. 1c and e)
Summary
Periodontitis is a chronic inflammatory disease in the surrounding tooth tissues [1] characterized by periodontal plaque microorganism-induced inflammation and loss of periodontal attachment [2]. The maintenance and restoration of oral tissue homeostasis after exposure to pathogens are essential to conquer oral inflammation, and the former depends on the complex coordination of innate and adaptive immune responses. In this regard, the evaluation and identification of tissue-specific immune cell types can help to illustrate the local immunoreactivity and severity of the inflammation. It is expected that the present study would provide useful information regarding the immune cell subpopulations in periodontitis, which could lead to the future control or prevention of periodontitis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
