Abstract
Human U251 and D54 glioma cells were tested for expression of 25 glioma-associated tumor antigen precursor proteins (TAPP) under hypoxic (1% O2) or normoxic (21% O2) conditions. Hypoxic glioma cell lines increased their mRNA expression for nine TAPP (Aim2, Art-4, EphA2, EZH2, Fosl1, PTH-rP, Sox 11, Whsc2 and YKL-40), as assessed by quantitative reverse transcriptase real-time/polymerase chain reaction (qRT-PCR). Increased differences with three hypoxic-induced TAPP: EZH2, Whsc2 and YKL-40 were shown at the protein levels by fluorescent antibody staining and quantitative electrophoretic analysis. Two TAPP (MRP3 and Trp1) were down-regulated by hypoxia in glioma cell lines. Growing the glioma cells under hypoxia for 13 days, followed by returning them back to normoxic conditions for 7 days, and restored the original normoxic TAPP profile. Thus, hypoxia was an environmental factor that stimulated the transient expression of these antigens. Intracranial xenografts grown in nude mice derived from U251 cells that had been cultured under neurosphere stem cell conditions showed increased expression of Whsc2 or YKL-40, demonstrating that these in vitro properties of glioma also occur in vivo. Whsc2-specific cytotoxic T lymphocytes killed the hypoxic U251 glioma cells better than normoxic glioma cells. The antigens expressed by hypoxic tumor cells may be a better source of starting tumor material for loading dendritic cells for novel immunotherapy of glioma using tumor-associated antigens.
Highlights
Normal atmosphere contains 21% oxygen (O2) (150 mm Hg is defined as normoxic)
No morphological changes were empirically observed with any cells when the U251 and D54 cells were grown under normoxic or 1% O2 conditions
Since U251 cells are HLA-0201 [33], we found a peptide that was derived from the Wolf-Hirschhorn syndrome candidate 2 (Whsc2) tumor antigen precursor proteins (TAPP) that could be recognized by CTLs via a HLA-0201 dependent restriction
Summary
Normal atmosphere contains 21% oxygen (O2) (150 mm Hg is defined as normoxic). Oxygenated blood leaving the lungs contains about 13% O2, while the blood returning to the lungs possesses about 5% O2 [1]–[4]. Due to irregular cellular vascularization in any cancer, there are various oxygen gradients within any tumor. The lack of oxygen regulates many genes and helps explain differences in tumor behavior reviewed in [8]–[11]. Extreme hypoxia kills glioma cells and helps explain the pseudo-palisading necrosis that is a hallmark characteristic of Glioblastoma Multiforme (GBM) [10]. It is concluded that oxygen tension between 0.5% and 2.5% is considered to be the best model of glioma hypoxia [8]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
