Differential gene expression profiles in the spontaneously hypertensive rats administrated with anti-hypertensive drugs (93.20)

Abstract

Abstract To identify and characterize the genes involved in the functional regulation of hypertension and arteriosclerosis-specific genes, changes in gene expression profiles were examined by DNA microarray from the spontaneously hypertensive rats (SHR) and normal the Wistar-Kyoto (WKY) rats after administration with anti-hypertension drugs, including angiotensin I-converting enzyme (ACE) inhibitor, enalapril and the calcium channel blocker, nifedifine. Among the genes that were distinct and differentially expressed in brain, heart and liver of SHR rats administrated with the drugs, 113 were specifically up-regulated, and 102 were down-regulated genes compared to those of WKY or SHR rats. The differential gene expression profiles in DNA microarray were corroborated by semi-quantitative RT-PCR. Of these genes, expression of TNFSF4, 6, 10, IL-6 receptor, IL-18 receptor accessory protein and CD24 genes was extensively exhibited in a hypertension-specific manner, and patterns for hypertension-associated gene expression was regulated by changes of their expression in angiotensin ¥± or H2O2-treated vascular smooth muscle cells. These results will provide a fundamental basis to delineate the novel mechanisms, and a thorough investigation of the relationship between their expression and function will provide a better understanding in the development of new therapeutic drugs against cardiovascular diseases. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2006-005-J03003)