Differential gene expression profile in ischemic myocardium of Wistar rats with acute myocardial infarction

Abstract

To determine the differential genes in ischemic myocardium of Wistar rats with acute myocardial infarction (AMI), we constructed two differential gene expression profiles. AMI model was generated by ligation of the left anterior descending coronary artery in Wistar rats. Total RNA was extracted from the normal and the ischemic heart tissues under the ligation point at the 8th day after the operation. Differential gene expression profiles of the two samples were constructed by using long serial analysis of gene expression (LongSAGE). Real time fluorescence quantitative PCR (Q-PCR) was used to confirm the expression changes of partial target genes. The main results were as follows: a total of 15966 tags were screened from the normal and the ischemic LongSAGE maps, and 9646 tags in the normal tissue and 9563 tags in the ischemic tissue were obtained. Among them, 7665 novel tags were identified by NCBI BLAST search. In the ischemic tissue, 142 genes significantly changed compared to those in the normal tissue (P<0.05). These differentially expressed genes may play important roles in the pathways of oxidation and phosphorylation, ATP synthesis and glycolysis and so on. Partial genes identified by the LongSAGE were confirmed by Q-PCR. The results show that AMI causes a series of gene expression changes in the regulation of the pathways related to energy metabolism.