Abstract
Gene expression profiles of Digitaria sanguinalis infected by Curvularia eragrostidis strain QZ-2000 at two concentrations of conidia and two dew durations were analyzed by cDNA amplified fragment length polymorphisms (cDNA-AFLP). Inoculum strength was more determinant of gene expression than dew duration. A total of 256 primer combinations were used for selective amplification and 1214 transcript-derived fragments (TDFs) were selected for their differential expression. Of these, 518 up-regulated differentially expressed TDFs were identified. Forty-six differential cDNA fragments were chosen to be cloned and 35 of them were successfully cloned and sequenced, of which 25 were homologous to genes of known function according to the GenBank database. Only 6 genes were up-regulated in Curvularia eragrostidis-inoculated D. sanguinalis, with functions involved in signal transduction, energy metabolism, cell growth and development, stress responses, abscisic acid biosynthesis and response. It appears that a few pathways may be important parts of the pathogenic strategy of C. eragrostidis strain QZ-2000 on D. sanguinalis. Our study provides the fundamentals to further study the pathogenic mechanism, screen for optimal C. eragrostidis strains as potential mycoherbicide and apply this product to control D. sanguinalis.
Highlights
Large crabgrass (Digitaria sanguinalis), is an annual plant with a great reproductive capacity that almost all cutting nodes with fibrous roots and buds can develop into new plants [1]
This strain is safe to most crops and economic plants, so it has the potential to be developed into a mycoherbicide against large crabgrass [12]
Effects of two spore concentrations and two dew durations on pathogenicity of QZ-2000 to D. sanguinalis were evaluated, and the differentially expressed genes of the infected plants were simultaneously detected by cDNA-AFLP
Summary
Large crabgrass (Digitaria sanguinalis), is an annual plant with a great reproductive capacity that almost all cutting nodes with fibrous roots and buds can develop into new plants [1]. Systematic evaluation of the pathogenicity, host specificity, and culture condition determined a good efficacy of C. eragrostidis when incubated at or above 26105 spore or more dosage and with dew for more than 24 hrs. This strain is safe to most crops and economic plants, so it has the potential to be developed into a mycoherbicide against large crabgrass [12]. Effects of two spore concentrations and two dew durations on pathogenicity of QZ-2000 to D. sanguinalis were evaluated, and the differentially expressed genes of the infected plants were simultaneously detected by cDNA-AFLP. The main objectives are to build gene expression profiles during C. eragrostidis infection of D. sanguinalis and elucidate the mechanism in this fungus infection and killing of this weed
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