Abstract

Previous studies on low temperature induction of flowering in Japanese radish, Raphanus sativa L., revealed that `Chinese Radish Jumbo Scarlet' was a cold obligate cultivar. `Chinese Radish Jumbo Scarlet' responded quantitatively to the number of days exposed to vernalizing temperatures (6 C). Fifteen percent of the plants were induced to flower when exposed to 6 C for 5 days. This response increased proportionally to 100% induction when plants were vernalized at 6 C for 30 days. This type of response allowed us to examine the changes in gene expression over time during the vernalization process.cDNA libraries were constructed from non-vernalized radish seedlings and seedlings vernalized for 1, 5, 15 or 30 days. Differential screening of the cDNA libraries was carried out to determine changes in gene expression related to the vernalization or induction processes. Up and down regulation of gene expression and activation or deactivation of genes during the vernalization process will be discussed. The ultimate goal of this project is to elucidate the processes involved in floral induction and how these processes are initiated by exposure of plants to vernalizing temperatures.

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