Differential gene expression analysis of in vitro duck hepatitis B virus infected primary duck hepatocyte cultures

Sajith Nair,Devaki S Arathy,Easwaran Sreekumar,Aneesh Issac

doi:10.1186/1743-422x-8-363

Sajith Nair, Devaki S Arathy + Show 2 more

Open Access

https://doi.org/10.1186/1743-422x-8-363

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Journal: Virology Journal	Publication Date: Jul 23, 2011
Citations: 29	License type: cc-by

Affiliation: Rajiv Gandhi Centre for Biotechnology

Abstract

BackgroundThe human hepatitis B virus (HBV), a member of the hepadna viridae, causes acute or chronic hepatitis B, and hepatocellular carcinoma (HCC). The duck hepatitis B virus (DHBV) infection, a dependable and reproducible model for hepadna viral studies, does not result in HCC unlike chronic HBV infection. Information on differential gene expression in DHBV infection might help to compare corresponding changes during HBV infection, and to delineate the reasons for this difference.FindingsA subtractive hybridization cDNA library screening of in vitro DHBV infected, cultured primary duck hepatocytes (PDH) identified cDNAs of 42 up-regulated and 36 down-regulated genes coding for proteins associated with signal transduction, cellular respiration, transcription, translation, ubiquitin/proteasome pathway, apoptosis, and membrane and cytoskeletal organization. Those coding for both novel as well as previously reported proteins in HBV/DHBV infection were present in the library. An inverse modulation of the cDNAs of ten proteins, reported to play role in human HCC, such as that of Y-box binding protein1, Platelet-activating factor acetylhydrolase isoform 1B, ribosomal protein L35a, Ferritin, α-enolase, Acid α-glucosidase and Caspase 3, copper-zinc superoxide dismutase (CuZnSOD), Filamin and Pyruvate dehydrogenase, was also observed in this in vitro study.ConclusionsThe present study identified cDNAs of a number of genes that are differentially modulated in in vitro DHBV infection of primary duck hepatocytes. Further correlation of this differential gene expression in in vivo infection models would be valuable to understand the little known aspects of the hepadnavirus biology.

Highlights

The human hepatitis B virus (HBV) and the duck hepatitis B virus (DHBV), which are members of the same virus family, hepadnaviridae, share several features in common [1]
The present study identified cDNAs of a number of genes that are differentially modulated in in vitro DHBV infection of primary duck hepatocytes
The virus infection was confirmed by PCR detection of a 300 bp DHBV glycoprotein 1 gene fragment in the DNA isolated from infected primary duck hepatocytes (PDH) culture supernatant (Figure 1B) and by sequence analysis

Summary

Introduction

The human hepatitis B virus (HBV) and the duck hepatitis B virus (DHBV), which are members of the same virus family, hepadnaviridae, share several features in common [1]. The establishment of the animal model with domestic duck employing the DHBV has helped greatly to overcome the shortcomings in HBV research [1,3] This model has its own limitations as revealed by the differences in the clinical manifestations of the disease in humans and birds infected by these viruses. This mainly pertains to the chronicity in DHBV infection without liver injury/hepatocellular carcinoma (HCC)/cirrhosis; spontaneous elimination of infection in adult ducks; and at the molecular level, the expression of only a cryptic X-protein [4]. Information on differential gene expression in DHBV infection might help to compare corresponding changes during HBV infection, and to delineate the reasons for this difference

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Conclusion