Abstract
The phytoplasma-associated grassy shoot disease is one of the major threats in sugarcane cultivation. A rapid method for enrichment and isolation of Sugarcane Grassy Shoot (SCGS) phytoplasma from the infected plants was developed. Differential filtration approach was used to isolate and enrich the SCGS phytoplasma and its genomic DNA that was detected by PCR analysis for phytoplasmal 16S rDNA. Ratio of pathogen to host plant DNA was found in the order of 103 and 105 in infected tissue and enriched fraction respectively, offering 148-fold increase in sensitivity for their detection.
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